Fall, 2006


Clinical Microbiology Syllabus

Clinical Laboratory Technician Program


Rose Dickinson

Meeting Times: Grade Percentages:
Home Phone:

 608.556.0017 (cell phone)

Required Texts:  Grade Components:



Lecture/Lab Schedule

 Health Occupations: 608.246.6065 Competencies: e-mail address
Cell Phone/e-mail You may call my cell phone at: 556-0017. In order to maintain my privacy please do not call after 9:00pm unless you consider the call to be urgent. My e-mail address is; I check my e-mail on a daily basis. 
MATC Closing: MATC School Hot-line #246-6606
Meeting Times: Wednesday Lecture:




Please note: lecture and lab times may change according to need.









Please note: lecture and lab times may change according to need.

Required Texts: 

  1. Diagnostic Microbiology, 2nd,W.B. Saunders Company, 2000

  2. Clinical Microbiology Laboratory Manual (provided)

  3. Optional: de la Maza, L.M., Pezzlo, M.T., and Baron, E.J., Color Atlas of Diagnostic Microbiology, 1st, Mosby, 1997 (good tool) Top 


To develop procedural skills in Clinical Microbiology along with the thinking processes that relate the following factors to each other.
  1. Patient characteristics
  2. Types of infections
  3. Specimen requirements
  4. Microscopic examinations
  5. Culture media: growth characteristics and amounts
  6. Specific organisms and their identification techniques.
  7. Treatment and procedures relating to that treatment. Top

  1. Describe and demonstrate the proper safety precautions while handling and disposing of infectious material including those potentially containing organisms like T. B., HIV or Hepatitis virus.

  2. Describe and perform proper specimen collection, transportation, and processing for bacterial (aerobic and anaerobic), and viral cultures.

  3. Perform common staining techniques of Gram stain, Acid fast stain, and lactophenol cotton blue directly from collected specimens or cultures and interpret the staining reactions and morphology microscopically.

  4. Select appropriate media for isolation of various pathogenic microorganisms from various body areas.

  5. Distinguish between pathogenic and nonpathogenic microorganisms on aerobic and anaerobic cultures from various body areas. 

  6. Select appropriate procedures for identifying the pathogens present in cultures, perform these procedures, and interpret the results accurately.

  7. Identify specimen requirements, usage, and techniques used for MIC's, Serum levels, beta lactamase, and Schlichter test.

  8. Identify how various antimicrobials work, which are useful against Gram positive and negative bacteria, Mycoplasma,  Chlamydia, and fungi, and specific restrictions when testing their usefulness.

  9. Relate the virulence factors of the microorganisms and host defense mechanisms to the resultant disease states.

  10. Perform and evaluate Kirby-Bauer sensitivity testing.

  11. Identify common types of nosocomial infections.

  12. Identify important components of the various isolation procedures utilized in hospitals and perform the procedures correctly when necessary.

  13. Perform and interpret quality control procedures for media, reagents, methods, and equipment and recognize potential problems occurring while performing tests or quality control procedures and take corrective action.

  14. Initiates questions showing an appreciation for the role of microbiology in relationship to the CLT profession and every day life.

  15. Conscientiously organizes and conducts microbiology activities in a manner that demonstrates concern for quick and accurate diagnosis and treatment of the patient's infection and concern for safety of fellow laboratory workers. Top


Evaluation Grading Policy
Grade Percentages 


A = 93 - 100%
AB = 89 - 92%
B = 85 - 88%
BC = 81 - 84%
C = 76 - 80%
D = 70 - 75%
F = BELOW 70% Top

Grade Components:
Study Guides for Types of Infection (Worksheets) 5%
Lecture/Lab Quizzes: (12 quizzes) 15%
Exams:  3/or 4 Unit Exams (approx. 50 points) & the Comprehensive Final (150 points) 30%

Lab Reports:  (20 lab reports X 5 points) 

Four Lab Practicals and Final Lab Practical 45%

Total Percentage:







Component Descriptions

Reading Assignments: Thirty four chapters of your text will be covered in this course.  In addition certain chapters in other texts will be duplicated as well as some NCCLS standards.  You will be responsible for reading all of these.  The class schedule indicates which reading must be done at which time.   The assigned readings are due before the topic is discussed in class.  Objectives and outlines are provided for each reading.   Return to Grade Components
Assessments, Quizzes, Exams and Final:


  • Study Guides for Types of Infection will be given as well as appropriate worksheets for content review. Return to Grade Components

  • Lecture/Lab Quizzes:  These short-answer quizzes will test your ability to read test results, gram stains, culture characteristics from photomicrographs.  On weeks when you have unknowns, you will determine what organism(s) are present in each students' unknowns as well as answering questions relating to the week's material. Your text, the optional atlas and the atlas from General Microbiology should help you perform well on these quizzes.  Return to Grade Components

  • Unit Exams: There will be three (or possibly four) unit exams. The test dates are on the attached schedule. There will be 45 to 50 multiple-choice questions. On occasion you may also be required to explain why your answer is the correct choice and why the others are incorrect.  To assist you in studying for these exams you have your learning objectives, chapter outlines, practice quizzes, sample questions and the case studies in the chapters. The testing procedure for these unit exams maximize the learning experience. You will take the test as usual with the answers recorded on both the scantron and the exam. Return to Grade Components

  • Final Exam: This is a cumulative final exam which will be held during exam week.  Return to Grade Components




Laboratory Work:

  • Lab reports:  These reports are a combination of computer-generated reports to the patients' charts and written "in-house" lab reports.  The computer-generated reports need to be maintained on a daily basis and the written reports need to be turned in by the Monday after receiving the knowns or unknowns.  They are an indication of your ability to match the expected results, but they will not be graded to complete accuracy since they are for practice.  The grade for these are determined by the following criteria:
    Turned in on time 5 points
    Deductions Not signed or dated by you -1
    Specimens miss identified or specimens mixed up -1
    Inappropriate choice of tests for organisms -1
    Inappropriate choice of susceptibility testing -1
    Late : -2.5
    Computer Report No Initial Report -0.5
    No Final Report -0.5
    Lab Report No Written Lab Report -1.5
    Not turned in: -5

       Return to Grade Components

  •  Laboratory Practicals: The laboratory practicals includes:  Culture plates with gram stains, rapid tests, and other identification tests covered during that unit.  They will test your ability to read gram stains, identification tests, recognize culture characteristics, identify organisms, correlate culture results with patient data and determine whether antimicrobic sensitivities need to be done.

 Return to Grade Components

Professional Behavior Evaluation:

I will give you a copy of the Professional Behavior Evaluation.  You will be requested to complete this form early in the semester and identify goals to work for the end of  the semester.  I will then review this during our academic advising session.  These are characteristics that will serve you well when you become a graduate CLT.

  Return to Grade Components

Lecture/Lab Schedule


Clinical Microbiology Syllabus